Background and Aim:  Effective brucellosis treatment necessitates antibiotics that can penetrate macrophages. This study systematic review and meta-analysis aimed at assessing the antibiotic SUSCEPTIBILITY profile of Brucella spp. isolated from humans, addressing this critical gap in understanding Brucella infections and their treatment. Materials and Methods:  Two authors conducted a systematic search across PubMed, Web of Science, and Scopus, with no time restrictions, encompassing English studies. Data extraction employed a standardized sheet, with two independent authors, and disagreements were resolved by a third. The data sheet included study characteristics and SUSCEPTIBILITY/resistance information for various antibiotics. Statistical analysis involved random effects models, Mantel-Haenszel methods, I2 tests, z-tests, and funnel plots using R and RStudio. Results:  Based on the pooled SUSCEPTIBILITY ratios, among the 630 samples of Brucella spp., 98% (95% CI: 85%-100%) were susceptible to trimethoprim. Among the 1255 samples of Brucella spp., 82% (95% CI: 54%-95%) were susceptible to rifampicin. Among the 1344 samples of Brucella spp., 100% (95% CI: 78%-100%) were susceptible to doxycycline. Among the 942 samples of Brucella spp., 100% (95% CI: 85%-100%) were susceptible to tetracycline. Among the 893 samples of Brucella spp., 100% (95% CI: 82%-100%) were susceptible to ciprofloxacin. Among the 906 samples of Brucella spp., 97% (95% CI: 96%-98%) were susceptible to gentamicin. Conclusion:  Based on our results, trimethoprim, doxycycline, tetracycline, ciprofloxacin, and gentamicin were effective, but rifampicin had lower SUSCEPTIBILITY. This informs antibiotic selection for Brucella infections, underscoring its importance in managing the disease

Background and Objectives: Ziehl-Neelsen stain is an initial route in diagnosis of pulmonary tuberculosis. Some studies show the sensitivity and specificity of the methods of liquid media culture in rapid diagnosis of the disease. In this study, we evaluated the correlation between Microscopic Observation Drug SUSCEPTIBILITY culture, Ziehl-Neelsen stain and Lowenstein-Jensen culture of sputum in suspected pulmonary tuberculosis patients.Material and Method: This was a descriptive study with the technique of observational-interview type. One hundred patients from Masih Daneshvari hospital were considered as pulmonary TB suspects. One early morning sputum sample was obtained from each patient and used for Ziehl-Neelsen stain, culture on Lowenstein-Jensen medium and MODS culture for drug SUSCEPTIBILITY testing.Results: On sputum examination, 40% of patients were smear positive by Ziehl-Neelsen stain, while 30% had positive sputum culture for Mycobacterium tuberculosis in Lowenstein-Jensen medium and 47% had positive MODS culture. This research showed a significant and direct correlation between MODS culture with Lowenstein-Jensen media (r=+0.39, P<0.0001). There was a significant association and a significant and reverse correlation between MODS culture with pleural effusion (r=-0.23, P<0.05). Multidrug resistance was observed in 55% of patients. Prevalence of positivity of these diagnostic methods among MDR patients was lower. There was a significant and direct correlation between MDR diseases with MODS culture. (r=+0.96 P<0.0001).Conclusion: due to increasing of MDR tuberculosis, rapid determination of resistance pattern is very important which is possible by using MODS cul ture method. However, since lower positivity of MODS culture is obtained in MDR patients, Lowenstein-Jensen culture method should be done as complementary method in suspected pulmonary tuberculosis patients.

Reports of fungal infections have increased over the past decades, making them a major threat to human health. In this study, we review the effects of genetic defects on SUSCEPTIBILITY to fungal diseases. To identify all relevant literature, we searched Google Scholar, PubMed, and Scopus and profiled studies published between 2008 and 2021. The results of several studies conducted on this subject have shown the significant effects of genetic variations such as hyper-IgE syndrome, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy syndrome, dectin-1 deficiency, CARD9 mutations, STAT1 mutations, and IL17 mutationson the host immune system’s response, which has an important impact on SUSCEPTIBILITY to fungal infections. The underlying immune system-related genetic profile affects the SUSCEPTIBILITY of individuals to different fungal infections,therefore, this subject should be further studied for better treatment of fungal diseases.